انتقال ژنهای مقاوم به بلاست Pi-1 و Pi-2به برنج رقم طارم دیلمانی

Rice cultivar Tarom Dilamani becauded a fragrance, flavor, cooking and marketing is a qualitative rice in Iran. This cultivar have high susceptibility against blast disease (Magnaporthe grisea). One of the important trouble producers of the Dilamani's rice cultivar is chemical control against blast disease and cause poisonous pollution of natural environment. The best manner in order to control this disease and avoid its damage is preparation and cultivation resistant cultivar. In this research we used classical breeding method to developing resistance cultivar. Female parent was qualitative Tarom Dilamani cultivar and male parent was near isogenic lines C101LAC and C101A51 that carry dominant resistant genes to blast Pi-1 and Pi-2, respectivly. Parents' seeds prepared, cultivated and propagated. When rice plant entered flowering stage, sensitive cultivar was confluenced with resistant lines' pollen. Hybrid seeds would cultivated next year. Plants cause selected according to morphological characters and virulences tests. Back cross was accomplished until four years until Bc4 generation. Plant inoculation was accomplished with dominant race of fungus cause blast at Agricultural Research Station of Sari Agricultural sciences and Natural Research university. Results of this research was transferred two resistance genes in sensitive Tarom Dilamani cultivar. The recovered cultivar showed resistance to races of fungus cause blast on region. Infection type of sensitivity (4 and 5) that was prior of accomplished of this research have converted to infection type of resistance (1 and 2). Morphology and quality of plant changed slightly, but was satisfactory prevention of damage due blast and increase grain yield.